Sequence-selective extraction of single-stranded DNA using DNA-functionalized reverse micelles.
نویسندگان
چکیده
We report here sequence-specific liquid/liquid extraction of single-stranded DNA using reverse micelles (water-in-oil microemulsions), in which hybridization between a DNA-surfactant and a target DNA having a complementary sequence allows selective transport of the target DNA to an organic phase from a mixture of DNA oligonucleotides.
منابع مشابه
Liquid-liquid extraction of enzymatically synthesized functional RNA oligonucleotides using reverse micelles with a DNA-surfactant.
We successfully implemented solvent extraction of short, single-stranded RNA using reverse micelles (water-in-oil microemulsions) with a DNA-surfactant. A thrombin-binding RNA aptamer was enzymatically synthesized and purified by extraction using the reverse micellar system. The extracted RNA aptamer retained thrombin-binding activity after the extraction procedure.
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عنوان ژورنال:
- Chemical communications
دوره 43 شماره
صفحات -
تاریخ انتشار 2007